The Organic and Biomimetic Chemistry Research Group is specialized in the design and synthesis of modified peptides and nucleic acids and methods for their conjugation and labeling.
More specifically major research interests include:
- The construction of conformationally defined peptide architectures. Scaffold decoration, cyclisation and peptide stapling are used to impose a particular conformation and stability on the parent peptides. Also, methods are being developed for the synthesis of both dipodal and tripodal peptides on solid phase. The synthesized compounds can possibly find applications as peptide vaccins, protein mimetics, DNA-binding ligands and artificial receptors or synthetic antibodies. More specifically the use of cholic acid based steroid derivatives has been explored for the conformational restriction and metabolic stabilization of appended peptide chains.
- The development of new methods for crosslinking of biomacromolecules such as peptides, proteins and oligonucleotides. Recently a very efficient furan-oxidation based crosslinking method has been developed for the site-selective labeling or introduction of covalent bonds between two binding partners.
- The design of novel reactive peptide and oligonucleotide based probes, including peptide nucleic acids, for applications in antisense and antigene strategies, protein and miRNA target identification and receptor pulldown.
Bioconjugated/modified Biomolecules: We develop new and modular synthetic derivatisation strategies, based on caged reactive groups, which can be activated on demand for further reaction with moieties containing groups with complementary reactivity. Methods are being developed for the synthesis of labeled proteins and nucleic acids, chemically derivatised proteins, protein-drug conjugates and various bioconjugates.
Solid phase synthesis of peptides and small heterocyclic peptidomimetics
- peptide synthesis, modification and conjugation
- methodologies for selective chemical modification of specific residues within peptides
- construction of conformationally restricted peptides
Solid phase synthesis of oligonucleotides and analogues
- oligonucleotide synthesis, modification and conjugation including DNA, RNA, 2’OMe RNA and PNA
- construction of reactive probes incorporating caged reactive functionalities for nucleic acid target identification
- antisense and antigene strategies
New labeling and conjugation methodologies
- synthesis of modified amino acids and nucleosides incorporating caged functionalities
- triggered activation by UV light, visible light or selective oxidation
Contact research group
Prof. Annemieke Madder
T +32 9 264 44 72Email: Annemieke.Madder@ugent.be